Kyle Root

Courses Most Commonly Taught

• Biochemistry I

Areas of Expertise

• Membrane protein expression/purification
• Membrane protein biophysics
• Protein labeling
• Lipases
• Membrane protein reconstitution into Lipidic Cubic Phase
• Protein refolding

Professional Publications

• Julien, Jeffrey A. et al. “One-step site-specific S-alkylation of full-length caveolin-1: Lipidation modulates the topology of its C-terminal domain.” Protein science. vol. 32,11 (2023): e4791. doi:10.1002/pro.4791
• Zachary Knepp, Ashlea Ghaner, and Kyle T. Root. “Purification and refolding protocol for cold-active recombinant esterase AaSGNH1 from Aphanizomenon flos-aquae expressed as insoluble inclusion bodies.” Prep Biochem Biotechnol. 2021 Aug 6:1-10.
• Monica D. Rieth, Kyle T. Root, and Kerney J. Glover. “Reconstitution of full-length human caveolin-1 into phospholipid bicelles: Validation by analytical ultracentrifugation.” Biophys. Chem. 259. (2020)
•  Kyle T. Root, Jeffery A. Julian, Kerney J. Glover. “Secondary Structure of Caveolins: A mini review.” Biochem. Soc. Trans. 31;47(5):1489-1498. (2019)
•  Tracy Caldwell, Svetlana Baoukina, Ashton T. Brock, Ryan C. Oliver, Kyle T. Root, Joanna K. Kreuger, Kerney J. Glover, Peter D. Tieleman, and Linda Columbus. “Low-q Bicelles Are Mixed Micelles.” J. Phys. Chem. Lett. 9(15). 4469-73. (2018)
•  Sarah M. Plucinsky, Kyle T. Root, and Kerney Jebrell Glover. “Efficient solubilization and purification of highly insoluble membrane proteins expressed as inclusion bodies using perfluorooctanoic acid.” Protein Expr. Purif. 143. 34-37. (2017)
•  Kyle T. Root, and Kerney Jebrell Glover. “Reconstitution and spectroscopic analysis of caveolin-1 residues 62-178 reveals that proline 110 governs its structure and solvent exposure.” Biochim. Biophys. Acta. 1858(4). 682-88. (2016)
• Kyle T. Root*, Sarah M. Plucinsky*, and Kerney Jebrell Glover, “Caveolin: Recent Progress in Structure, Topology, and Oligomeric Behavior” Curr. Top. Membr. 75. 305-36. (2015)
•  Kyle T. Root. Reconstitution and Topological Analysis of Caveolin-1 in Model Membrane Systems. Doctoral Thesis. Lehigh University 2015.
•  Kyle T. Root*, Huan Rui*, Jinwoo Lee, Kerney Jebrell Glover, Wonpil Im. “Probing the U-Shaped Conformation of Caveolin-1 in a Bilayer.” Biophys J. 106(6). 1371-80. (2014)
• Cheyenne Mckibbin, Kyle T. Root, Gregory Carson. “Metal Ion Concentration within Algal Tiusse of Species Growing in Proximity to Tioga River Outflows Affected by Acid Mine Drainage in Northern Pennsylvania.” J. Undergrad. Res. 9. 17-22. (2009)